Human PDK1 activation kit by CRISPRa

CAT#: GA103460

PDK1 CRISPRa kit - CRISPR gene activation of human pyruvate dehydrogenase kinase 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: PDK1
• Locus ID: 5163
• Kit Components:

  GA103460G1, PDK1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103460G2, PDK1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103460G3, PDK1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001278549, NM_002610, NR_103729, NR_103731
• Summary: 'Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2013]'