Human PFKFB2 activation kit by CRISPRa

CAT#: GA103498

PFKFB2 CRISPRa kit - CRISPR gene activation of human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: PFKFB2
• Locus ID: 5208
• Kit Components:

  GA103498G1, PFKFB2 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103498G2, PFKFB2 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103498G3, PFKFB2 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001018053, NM_006212
• Synonyms: PFK-2/FBPase-2
• Summary: 'The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'