Human PKC zeta (PRKCZ) activation kit by CRISPRa

CAT#: GA103780

PRKCZ CRISPRa kit - CRISPR gene activation of human protein kinase C zeta

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: PRKCZ
• Locus ID: 5590
• Kit Components:

  GA103780G1, PKC zeta gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103780G2, PKC zeta gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103780G3, PKC zeta gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001033581, NM_001033582, NM_001242874, NM_002744, NM_001350803, NM_001350804, NM_001350805, NM_001350806, NR_146911
• Synonyms: PKC-ZETA; PKC2
• Summary: 'Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]'