Human ROCK1 activation kit by CRISPRa

CAT#: GA104128

ROCK1 CRISPRa kit - CRISPR gene activation of human Rho associated coiled-coil containing protein kinase 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: ROCK1
• Locus ID: 6093
• Kit Components:

  GA104128G1, ROCK1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA104128G2, ROCK1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA104128G3, ROCK1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_005406
• Synonyms: P160ROCK; ROCK-I
• Summary: 'This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18. [provided by RefSeq, Aug 2015]'