Human MTGR1 (CBFA2T2) activation kit by CRISPRa

CAT#: GA106091

CBFA2T2 CRISPRa kit - CRISPR gene activation of human CBFA2/RUNX1 partner transcriptional co-repressor 2

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: CBFA2T2
• Locus ID: 9139
• Kit Components:

  GA106091G1, CBFA2T2 gRNA vector 1 in pCas-Guide-CRISPRa

  GA106091G2, CBFA2T2 gRNA vector 2 in pCas-Guide-CRISPRa

  GA106091G3, CBFA2T2 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001032999, NM_001039709, NM_005093, NM_175864
• Synonyms: EHT; MTGR1; p85; ZMYND3
• Summary: In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the 3'-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several transcript variants are thought to exist for this gene, but the full-length natures of only three have been described. [provided by RefSeq, Jul 2008]