Human PATZ1 activation kit by CRISPRa

CAT#: GA108426

PATZ1 CRISPRa kit - CRISPR gene activation of human POZ/BTB and AT hook containing zinc finger 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: PATZ1
• Locus ID: 23598
• Kit Components:

  GA108426G1, PATZ1 gRNA vector 1 in pCas-Guide-CRISPRa

  GA108426G2, PATZ1 gRNA vector 2 in pCas-Guide-CRISPRa

  GA108426G3, PATZ1 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_014323, NM_032050, NM_032051, NM_032052
• Synonyms: dJ400N23; MAZR; PATZ; RIAZ; ZBTB19; ZNF278; ZSG
• Summary: The protein encoded by this gene contains an A-T hook DNA binding motif which usually binds to other DNA binding structures to play an important role in chromatin modeling and transcription regulation. Its Poz domain is thought to function as a site for protein-protein interaction and is required for transcriptional repression, and the zinc-fingers comprise the DNA binding domain. Since the encoded protein has typical features of a transcription factor, it is postulated to be a repressor of gene expression. In small round cell sarcoma, this gene is fused to EWS by a small inversion of 22q, then the hybrid is thought to be translocated (t(1;22)(p36.1;q12). The rearrangement of chromosome 22 involves intron 8 of EWS and exon 1 of this gene creating a chimeric sequence containing the transactivation domain of EWS fused to zinc finger domain of this protein. This is a distinct example of an intra-chromosomal rearrangement of chromosome 22. Four alternatively spliced transcript variants are described for this gene. [provided by RefSeq, Jul 2008]