Human APEX2 activation kit by CRISPRa

CAT#: GA109110

APEX2 CRISPRa kit - CRISPR gene activation of human apurinic/apyrimidinic endodeoxyribonuclease 2

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: APEX2
• Locus ID: 27301
• Kit Components:

  GA109110G1, APEX2 gRNA vector 1 in pCas-Guide-CRISPRa

  GA109110G2, APEX2 gRNA vector 2 in pCas-Guide-CRISPRa

  GA109110G3, APEX2 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001271748, NM_014481
• Synonyms: APE2; APEXL2; XTH2; ZGRF2
• Summary: Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes a protein shown to have a weak class II AP endonuclease activity. Most of the encoded protein is located in the nucleus but some is also present in mitochondria. This protein may play an important role in both nuclear and mitochondrial base excision repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012]