Human DROSHA activation kit by CRISPRa

CAT#: GA109235

DROSHA CRISPRa kit - CRISPR gene activation of human drosha ribonuclease III

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: DROSHA
• Locus ID: 29102
• Kit Components:

  GA109235G1, DROSHA gRNA vector 1 in pCas-Guide-CRISPRa

  GA109235G2, DROSHA gRNA vector 2 in pCas-Guide-CRISPRa

  GA109235G3, DROSHA gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001100412, NM_013235
• Synonyms: ETOHI2; HSA242976; RANSE3L; RN3; RNASE3L; RNASEN
• Summary: This gene encodes a ribonuclease (RNase) III double-stranded RNA-specific ribonuclease and subunit of the microprocessor protein complex, which catalyzes the initial processing step of microRNA (miRNA) synthesis. The encoded protein cleaves the stem loop structure from the primary microRNA (pri-miRNA) in the nucleus, yielding the precursor miRNA (pre-miRNA), which is then exported to the cytoplasm for further processing. In a human cell line lacking a functional copy of this gene, canonical miRNA synthesis is reduced. Somatic mutations in this gene have been observed in human patients with kidney cancer. [provided by RefSeq, Sep 2016]