Human A1CF activation kit by CRISPRa

CAT#: GA109352

A1CF CRISPRa kit - CRISPR gene activation of human APOBEC1 complementation factor

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: A1CF
• Locus ID: 29974
• Kit Components:

  GA109352G1, A1CF gRNA vector 1 in pCas-Guide-CRISPRa

  GA109352G2, A1CF gRNA vector 2 in pCas-Guide-CRISPRa

  GA109352G3, A1CF gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001198818, NM_001198819, NM_001198820, NM_014576, NM_138932, NM_138933, NM_001370130, NM_001370131
• Synonyms: ACF; ACF64; ACF65; APOBEC1CF; ASP
• Summary: Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]