Human ZBTB4 activation kit by CRISPRa

CAT#: GA111665

ZBTB4 CRISPRa kit - CRISPR gene activation of human zinc finger and BTB domain containing 4

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: ZBTB4
• Locus ID: 57659
• Kit Components:

  GA111665G1, ZBTB4 gRNA vector 1 in pCas-Guide-CRISPRa

  GA111665G2, ZBTB4 gRNA vector 2 in pCas-Guide-CRISPRa

  GA111665G3, ZBTB4 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001128833, NM_020899
• Synonyms: KAISO-L1; ZNF903
• Summary: Transcriptional repressor with bimodal DNA-binding specificity. Represses transcription in a methyl-CpG-dependent manner. Binds with a higher affinity to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' but can also bind to the non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). Can also bind specifically to a single methyl-CpG pair and can bind hemimethylated DNA but with a lower affinity compared to methylated DNA (PubMed:16354688). Plays a role in postnatal myogenesis, may be involved in the regulation of satellite cells self-renewal. [UniProtKB/Swiss-Prot Function]