Human MANEA activation kit by CRISPRa

CAT#: GA112554

MANEA CRISPRa kit - CRISPR gene activation of human mannosidase endo-alpha

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: MANEA
• Locus ID: 79694
• Kit Components:

  GA112554G1, MANEA gRNA vector 1 in pCas-Guide-CRISPRa

  GA112554G2, MANEA gRNA vector 2 in pCas-Guide-CRISPRa

  GA112554G3, MANEA gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_024641
• Synonyms: ENDO; hEndo
• Summary: N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005 [PubMed 15677381]). [supplied by OMIM, Sep 2008]