Human UNC45B activation kit by CRISPRa

CAT#: GA115588

UNC45B CRISPRa kit - CRISPR gene activation of human unc-45 myosin chaperone B

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: UNC45B
• Locus ID: 146862
• Kit Components:

  GA115588G1, UNC45B gRNA vector 1 in pCas-Guide-CRISPRa

  GA115588G2, UNC45B gRNA vector 2 in pCas-Guide-CRISPRa

  GA115588G3, UNC45B gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001033576, NM_001267052, NM_001308281, NM_173167
• Synonyms: CMYA4; CTRCT43; SMUNC45; UNC-45B; UNC45
• Summary: This gene encodes a co-chaperone required for folding and accumulation of type II myosins. The protein consists of three tetratricopeptide repeat motifs at the N-terminus that form a complex with heat shock protein 90, a central region of unknown function that is conserved in all Unc-45 proteins, and a C-terminal Unc-45/Cro1/She4 domain. The protein is expressed at high levels in striated muscle, where its muscle myosin chaperone activity is dependent on heat shock protein 90 acting as a co-chaperone. A missense mutation in this gene has been associated with cataract development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]