Mouse Rnaseh1 activation kit by CRISPRa

CAT#: GA203651

Rnaseh1 CRISPRa kit - CRISPR gene activation of mouse ribonuclease H1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Rnaseh1
• Locus ID: 19819
• Kit Components:

  GA203651G1, Rnaseh1 gRNA vector 1 in pCas-Guide-CRISPRa

  GA203651G2, Rnaseh1 gRNA vector 2 in pCas-Guide-CRISPRa

  GA203651G3, Rnaseh1 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001286865, NM_011275, NR_104608
• Summary: This gene encodes an endonuclease that specifically degrades the RNA of RNA-DNA hybrids and is necessary for DNA replication and repair. This enzyme is present in both mitochondria and nuclei, which are resulted from translation of a single mRNA with two in-frame initiation start codons. The use of the first start codon produces the mitochondrial isoform and the use of the second start codon produces the nuclear isoform. The production of the mitochondrial isoform is modulated by an upstream open reading frame (uORF) which encodes 7aa in mouse. An alternately spliced transcript variant has been found which is a candidate for nonsense-mediated mRNA decay (NMD). [provided by RefSeq, Nov 2013]