Mouse Mdc1 activation kit by CRISPRa

CAT#: GA214902

Mdc1 CRISPRa kit - CRISPR gene activation of mouse mediator of DNA damage checkpoint 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Mdc1
• Locus ID: 240087
• Kit Components:

  GA214902G1, Mdc1 gRNA vector 1 in pCas-Guide-CRISPRa

  GA214902G2, Mdc1 gRNA vector 2 in pCas-Guide-CRISPRa

  GA214902G3, Mdc1 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001010833
• Synonyms: 6820401C03; AA413496; mKIAA0170; Nfbd1
• Summary: The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 7 divergent copies of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. Mice with mutations in this gene exhibit growth retardation, male infertility, immune defects, chromosome instability, DNA repair defects, and radiation sensitivity. [provided by RefSeq, Jul 2008]