Mouse Lefty2 activation kit by CRISPRa

CAT#: GA216444

Lefty2 CRISPRa kit - CRISPR gene activation of mouse left-right determination factor 2

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Lefty2
• Locus ID: 320202
• Kit Components:

  GA216444G1, Lefty2 gRNA vector 1 in pCas-Guide-CRISPRa

  GA216444G2, Lefty2 gRNA vector 2 in pCas-Guide-CRISPRa

  GA216444G3, Lefty2 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_177099
• Synonyms: 6030463A22Rik; Ebaf; Lefta; Leftb; lefty-2; lefty-B
• Summary: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which plays a role in left-right asymmetry determination of organ systems during development. This protein is also important in self-renewal and differentiation of mouse embryonic stem cells. Mice lacking a functional copy of this gene exhibit defects in axial and left-right patterning. [provided by RefSeq, Aug 2016]