PNPO (NM_018129) Human Mass Spec Standard

CAT#: PH300133

PNPO MS Standard C13 and N15-labeled recombinant protein (NP_060599)

Product Images

Coomassie blue staining of purified PNPO protein (Cat# TP300133). The protein was produced from HEK293T cells transfected with PNPO cDNA clone (Cat# RC200133) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC200133
• Predicted MW: 30 kDa
• Protein Sequence:
  Sequence Link

  >RC200133 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTCWLRGVTATFGRPAEWPGYLSHLCGRSAAMDLGPMRKSYRGDREAFEETHLTSLDPVKQFAAWFEEAV
  QCPDIGEANAMCLATCTRDGKPSARMLLLKGFGKDGFRFFTNFESRKGKELDSNPFASLVFYWEPLNRQV
  RVEGPVKKLPEEEAECYFHSRPKSSQIGAVVSHQSSVIPDREYLRKKNEELEQLYQDQEVPKPKSWGGYV
  LYPQVMEFWQGQTNRLHDRIVFRRGLPTGDSPLGPMTHRGEEDWLYERLAP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_060599
• RefSeq Size: 3482
• RefSeq ORF: 783
• Synonyms: HEL-S-302; PDXPO
• Locus ID: 55163
• UniProt ID: Q9NVS9, V9HW45
• Cytogenetics: 17q21.32
• Summary: The enzyme encoded by this gene catalyzes the terminal, rate-limiting step in the synthesis of pyridoxal 5'-phosphate, also known as vitamin B6. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine. Mutations in this gene result in pyridoxamine 5'-phosphate oxidase (PNPO) deficiency, a form of neonatal epileptic encephalopathy. [provided by RefSeq, Oct 2008]
• Protein Pathways: Metabolic pathways, Vitamin B6 metabolism