PNPO (NM_018129) Human Tagged ORF Clone
CAT#: RC200133
PNPO (Myc-DDK-tagged)-Human pyridoxamine 5'-phosphate oxidase (PNPO)
"NM_018129" in other vectors (7)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | PNPO |
Synonyms | HEL-S-302; PDXPO |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC200133 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGTGCTGGCTGCGGGGCGTCACGGCGACGTTCGGGCGACCTGCCGAGTGGCCAGGCTACCTCAGTC ACCTGTGTGGTCGCAGTGCTGCCATGGACCTGGGACCCATGCGCAAGAGTTACCGCGGGGACCGAGAGGC ATTTGAGGAGACTCATCTGACCTCTCTTGACCCAGTGAAACAGTTTGCTGCCTGGTTTGAGGAGGCTGTT CAGTGTCCTGACATAGGGGAAGCCAATGCCATGTGTCTGGCTACCTGCACCAGAGATGGAAAACCCTCTG CTCGCATGTTGCTGCTGAAGGGCTTCGGGAAAGATGGCTTCCGCTTCTTCACTAACTTCGAGAGTCGAAA AGGAAAAGAGCTGGACTCTAATCCCTTTGCTTCCCTTGTCTTCTACTGGGAGCCACTTAACCGTCAGGTG CGTGTGGAAGGCCCTGTGAAGAAACTGCCTGAGGAGGAGGCTGAGTGCTACTTCCACTCCCGCCCCAAGA GCAGCCAGATTGGGGCTGTGGTCAGCCACCAGAGTTCTGTGATCCCTGATCGGGAGTATCTGAGAAAGAA AAATGAGGAACTGGAACAGCTCTACCAGGATCAAGAGGTGCCCAAGCCAAAATCCTGGGGTGGCTATGTC CTGTACCCTCAGGTGATGGAGTTCTGGCAAGGTCAAACCAACCGCCTGCATGACCGGATAGTCTTTCGGC GGGGCCTACCCACAGGAGATTCCCCTTTGGGGCCCATGACCCACCGCGGGGAGGAAGACTGGCTCTATGA GAGACTTGCACCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200133 protein sequence
Red=Cloning site Green=Tags(s) MTCWLRGVTATFGRPAEWPGYLSHLCGRSAAMDLGPMRKSYRGDREAFEETHLTSLDPVKQFAAWFEEAV QCPDIGEANAMCLATCTRDGKPSARMLLLKGFGKDGFRFFTNFESRKGKELDSNPFASLVFYWEPLNRQV RVEGPVKKLPEEEAECYFHSRPKSSQIGAVVSHQSSVIPDREYLRKKNEELEQLYQDQEVPKPKSWGGYV LYPQVMEFWQGQTNRLHDRIVFRRGLPTGDSPLGPMTHRGEEDWLYERLAP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_018129 |
ORF Size | 783 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_018129.1, NM_018129.2, NM_018129.3, NP_060599.1 |
RefSeq Size | 3482 bp |
RefSeq ORF | 786 bp |
Locus ID | 55163 |
Domains | Pyridox_oxidase |
Protein Pathways | Metabolic pathways, Vitamin B6 metabolism |
MW | 30 kDa |
Gene Summary | The enzyme encoded by this gene catalyzes the terminal, rate-limiting step in the synthesis of pyridoxal 5'-phosphate, also known as vitamin B6. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine. Mutations in this gene result in pyridoxamine 5'-phosphate oxidase (PNPO) deficiency, a form of neonatal epileptic encephalopathy. [provided by RefSeq, Oct 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC108655 | PNPO (untagged)-Human pyridoxamine 5'-phosphate oxidase (PNPO) |
USD 310.00 |
|
SC320715 | PNPO (untagged)-Human pyridoxamine 5'-phosphate oxidase (PNPO) |
USD 310.00 |
|
RG200133 | PNPO (GFP-tagged) - Human pyridoxamine 5'-phosphate oxidase (PNPO) |
USD 460.00 |
|
RC200133L1 | Lenti ORF clone of Human pyridoxamine 5'-phosphate oxidase (PNPO), Myc-DDK-tagged |
USD 768.00 |
|
RC200133L2 | Lenti ORF clone of Human pyridoxamine 5'-phosphate oxidase (PNPO), mGFP tagged |
USD 768.00 |
|
RC200133L3 | Lenti ORF clone of Human pyridoxamine 5'-phosphate oxidase (PNPO), Myc-DDK-tagged |
USD 768.00 |
|
RC200133L4 | Lenti ORF clone of Human pyridoxamine 5'-phosphate oxidase (PNPO), mGFP tagged |
USD 768.00 |
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