PNPO (NM_018129) Human Tagged ORF Clone

CAT#: RG200133

PNPO (GFP-tagged) - Human pyridoxamine 5'-phosphate oxidase (PNPO)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PNPO
• Synonyms: HEL-S-302; PDXPO
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200133 representing NM_018129
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGTGCTGGCTGCGGGGCGTCACGGCGACGTTCGGGCGACCTGCCGAGTGGCCAGGCTACCTCAGTC
  ACCTGTGTGGTCGCAGTGCTGCCATGGACCTGGGACCCATGCGCAAGAGTTACCGCGGGGACCGAGAGGC
  ATTTGAGGAGACTCATCTGACCTCTCTTGACCCAGTGAAACAGTTTGCTGCCTGGTTTGAGGAGGCTGTT
  CAGTGTCCTGACATAGGGGAAGCCAATGCCATGTGTCTGGCTACCTGCACCAGAGATGGAAAACCCTCTG
  CTCGCATGTTGCTGCTGAAGGGCTTCGGGAAAGATGGCTTCCGCTTCTTCACTAACTTCGAGAGTCGAAA
  AGGAAAAGAGCTGGACTCTAATCCCTTTGCTTCCCTTGTCTTCTACTGGGAGCCACTTAACCGTCAGGTG
  CGTGTGGAAGGCCCTGTGAAGAAACTGCCTGAGGAGGAGGCTGAGTGCTACTTCCACTCCCGCCCCAAGA
  GCAGCCAGATTGGGGCTGTGGTCAGCCACCAGAGTTCTGTGATCCCTGATCGGGAGTATCTGAGAAAGAA
  AAATGAGGAACTGGAACAGCTCTACCAGGATCAAGAGGTGCCCAAGCCAAAATCCTGGGGTGGCTATGTC
  CTGTACCCTCAGGTGATGGAGTTCTGGCAAGGTCAAACCAACCGCCTGCATGACCGGATAGTCTTTCGGC
  GGGGCCTACCCACAGGAGATTCCCCTTTGGGGCCCATGACCCACCGCGGGGAGGAAGACTGGCTCTATGA
  GAGACTTGCACCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200133 representing NM_018129
  Red=Cloning site Green=Tags(s)
  
  MTCWLRGVTATFGRPAEWPGYLSHLCGRSAAMDLGPMRKSYRGDREAFEETHLTSLDPVKQFAAWFEEAV
  QCPDIGEANAMCLATCTRDGKPSARMLLLKGFGKDGFRFFTNFESRKGKELDSNPFASLVFYWEPLNRQV
  RVEGPVKKLPEEEAECYFHSRPKSSQIGAVVSHQSSVIPDREYLRKKNEELEQLYQDQEVPKPKSWGGYV
  LYPQVMEFWQGQTNRLHDRIVFRRGLPTGDSPLGPMTHRGEEDWLYERLAP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018129
• ORF Size: 783 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_018129.2, NP_060599.1
• RefSeq Size: 3437
• RefSeq ORF: 786
• Locus ID: 55163
• Domains: Pyridox_oxidase
• Protein Pathways: Metabolic pathways, Vitamin B6 metabolism
• Gene Summary: The enzyme encoded by this gene catalyzes the terminal, rate-limiting step in the synthesis of pyridoxal 5'-phosphate, also known as vitamin B6. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine. Mutations in this gene result in pyridoxamine 5'-phosphate oxidase (PNPO) deficiency, a form of neonatal epileptic encephalopathy. [provided by RefSeq, Oct 2008]