ASCL1 (NM_004316) Human Mass Spec Standard

CAT#: PH301123

ASCL1 MS Standard C13 and N15-labeled recombinant protein (NP_004307)

Product Images

Coomassie blue staining of purified ASCL1 protein (Cat# TP301123). The protein was produced from HEK293T cells transfected with ASCL1 cDNA clone (Cat# RC201123) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC201123
• Predicted MW: 25.5 kDa
• Protein Sequence:
  Sequence Link

  >RC201123 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MESSAKMESGGAGQQPQPQPQQPFLPPAACFFATAAAAAAAAAAAAAQSAQQQQQQQQQQQQAPQLRPAA
  DGQPSGGGHKSAPKQVKRQRSSSPELMRCKRRLNFSGFGYSLPQQQPAAVARRNERERNRVKLVNLGFAT
  LREHVPNGAANKKMSKVETLRSAVEYIRALQQLLDEHDAVSAAFQAGVLSPTISPNYSNDLNSMAGSPVS
  SYSSDEGSYDPLSPEEQELLDFTNWF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_004307
• RefSeq Size: 2490
• RefSeq ORF: 708
• Synonyms: ASH1; bHLHa46; HASH1; MASH1
• Locus ID: 429
• UniProt ID: P50553
• Cytogenetics: 12q23.2
• Summary: 'This gene encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases. [provided by RefSeq, Jul 2008]'
• Protein Families: Druggable Genome, Transcription Factors