PSMA7 (NM_002792) Human Mass Spec Standard

CAT#: PH301169

PSMA7 MS Standard C13 and N15-labeled recombinant protein (NP_002783)

Product Images

Coomassie blue staining of purified PSMA7 protein (Cat# TP301169). The protein was produced from HEK293T cells transfected with PSMA7 cDNA clone (Cat# RC201169) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC201169
• Predicted MW: 27.9 kDa
• Protein Sequence:
  Sequence Link

  >RC201169 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSYDRAITVFSPDGHLFQVEYAQEAVKKGSTAVGVRGRDIVVLGVEKKSVAKLQDERTVRKICALDDNVC
  MAFAGLTADARIVINRARVECQSHRLTVEDPVTVEYITRYIASLKQRYTQSNGRRPFGISALIVGFDFDG
  TPRLYQTDPSGTYHAWKANAIGRGAKSVREFLEKNYTDEAIETDDLTIKLVIKALLEVVQSGGKNIELAV
  MRRDQSLKILNPEEIEKYVAEIEKEKEENEKKKQKKAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_002783
• RefSeq Size: 1050
• RefSeq ORF: 744
• Synonyms: C6; HEL-S-276; HSPC; RC6-1; XAPC7
• Locus ID: 5688
• UniProt ID: O14818, A0A0K0K1K4
• Cytogenetics: 20q13.33
• Summary: 'The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the peptidase T1A family that functions as a 20S core alpha subunit. The encoded protein interacts with the hepatitis B virus X protein and plays a role in regulating hepatitis C virus internal ribosome entry site (IRES) activity, an activity essential for viral replication. The encoded protein also plays a role in the cellular stress response by regulating hypoxia-inducible factor-1alpha. A pseudogene of this gene is located on the long arm of chromosome 9. [provided by RefSeq, Jul 2012]'
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Proteasome