ILKAP (NM_030768) Human Mass Spec Standard

CAT#: PH301456

ILKAP MS Standard C13 and N15-labeled recombinant protein (NP_110395)

Product Images

Coomassie blue staining of purified ILKAP protein (Cat# TP301456). The protein was produced from HEK293T cells transfected with ILKAP cDNA clone (Cat# RC201456) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC201456
• Predicted MW: 42.9 kDa
• Protein Sequence:
  Sequence Link

  >RC201456 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDLFGDLPEPERSPRPAAGKEAQKGPLLFDDLPPASSTDSGSGGPLLFDDLPPASSGDSGSLATSISQMV
  KTEGKGAKRKTSEEEKNGSEELVEKKVCKASSVIFGLKGYVAERKGEREEMQDAHVILNDITEECRPPSS
  LITRVSYFAVFDGHGGIRASKFAAQNLHQNLIRKFPKGDVISVEKTVKRCLLDTFKHTDEEFLKQASSQK
  PAWKDGSTATCVLAVDNILYIANLGDSRAILCRYNEESQKHAALSLSKEHNPTQYEERMRIQKAGGNVRD
  GRVLGVLEVSRSIGDGQYKRCGVTSVPDIRRCQLTPNDRFILLACDGLFKVFTPEEAVNFILSCLEDEKI
  QTREGKSAADARYEAACNRLANKAVQRGSADNVTVMVVRIGH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_110395
• RefSeq Size: 1480
• RefSeq ORF: 1176
• Synonyms: ILKAP2; ILKAP3; PP2C-DELTA; PPM1O
• Locus ID: 80895
• UniProt ID: Q9H0C8
• Cytogenetics: 2q37.3
• Summary: The protein encoded by this gene is a protein serine/threonine phosphatase of the PP2C family. This protein can interact with integrin-linked kinase (ILK/ILK1), a regulator of integrin mediated signaling, and regulate the kinase activity of ILK. Through the interaction with ILK, this protein may selectively affect the signaling process of ILK-mediated glycogen synthase kinase 3 beta (GSK3beta), and thus participate in Wnt signaling pathway. [provided by RefSeq, Jul 2008]
• Protein Families: Druggable Genome, Phosphatase