HADHSC (HADH) (NM_005327) Human Mass Spec Standard

CAT#: PH301752

HADH MS Standard C13 and N15-labeled recombinant protein (NP_005318)

Product Images

Coomassie blue staining of purified HADH protein (Cat# TP301752). The protein was produced from HEK293T cells transfected with HADH cDNA clone (Cat# RC201752) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC201752
• Predicted MW: 34.3 kDa
• Protein Sequence:
  Sequence Link

  >RC201752 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAFVTRQFMRSVSSSSTASASAKKIIVKHVTVIGGGLMGAGIAQVAAATGHTVVLVDQTEDILAKSKKGI
  EESLRKVAKKKFAENPKAGDEFVEKTLSTIATSTDAASVVHSTDLVVEAIVENLKVKNELFKRLDKFAAE
  HTIFASNTSSLQITSIANATTRQDRFAGLHFFNPVPVMKLVEVIKTPMTSQKTFESLVDFSKALGKHPVS
  CKDTPGFIVNRLLVPYLMEAIRLYERGDASKEDIDTAMKLGAGYPMGPFELLDYVGLDTTKFIVDGWHEM
  DAENPLHQPSPSLNKLVAENKFGKKTGEGFYKYK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_005318
• RefSeq Size: 1986
• RefSeq ORF: 942
• Synonyms: HAD; HADH1; HADHSC; HCDH; HHF4; MSCHAD; SCHAD
• Locus ID: 3033
• UniProt ID: Q16836, A0A140VK76
• Cytogenetics: 4q25
• Summary: 'This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene of this gene on chromosome 15. [provided by RefSeq, May 2010]'
• Protein Pathways: Butanoate metabolism, Fatty acid elongation in mitochondria, Fatty acid metabolism, Lysine degradation, Metabolic pathways, Tryptophan metabolism, Valine, leucine and isoleucine degradation