Carbonic Anhydrase II (CA2) (NM_000067) Human Mass Spec Standard

CAT#: PH301974

CA2 MS Standard C13 and N15-labeled recombinant protein (NP_000058)

Product Images

Coomassie blue staining of purified CA2 protein (Cat# TP301974). The protein was produced from HEK293T cells transfected with CA2 cDNA clone (Cat# RC201974) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC201974
• Predicted MW: 29.2 kDa
• Protein Sequence:
  Sequence Link

  >RC201974 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSHHWGYGKHNGPEHWHKDFPIAKGERQSPVDIDTHTAKYDPSLKPLSVSYDQATSLRILNNGHAFNVEF
  DDSQDKAVLKGGPLDGTYRLIQFHFHWGSLDGQGSEHTVDKKKYAAELHLVHWNTKYGDFGKAVQQPDGL
  AVLGIFLKVGSAKPGLQKVVDVLDSIKTKGKSADFTNFAARGLLPESLDYWTYPGSLTTPPLLECVTWIV
  LKEPISVSSEQVLKFRKLNFNGEGEPEELMVDNWRPAQPLKNRQIKASFK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_000058
• RefSeq Size: 1666
• RefSeq ORF: 780
• Synonyms: CA-II; CAC; CAII; Car2; HEL-76; HEL-S-282
• Locus ID: 760
• UniProt ID: P00918, V9HW21
• Cytogenetics: 8q21.2
• Summary: 'The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]'
• Protein Families: Druggable Genome
• Protein Pathways: Nitrogen metabolism