SPINT2 (NM_021102) Human Mass Spec Standard

CAT#: PH302044

SPINT2 MS Standard C13 and N15-labeled recombinant protein (NP_066925)

Product Images

Coomassie blue staining of purified SPINT2 protein (Cat# TP302044). The protein was produced from HEK293T cells transfected with SPINT2 cDNA clone (Cat# RC202044) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC202044
• Predicted MW: 28.2 kDa
• Protein Sequence:
  Sequence Link

  >RC202044 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAQLCGLRRSRAFLALLGSLLLSGVLAADRERSIHDFCLVSKVVGRCRASMPRWWYNVTDGSCQLFVYGG
  CDGNSNNYLTKEECLKKCATVTENATGDLATSRNAADSSVPSAPRRQDSEDHSSDMFNYEEYCTANAVTG
  PCRASFPRWYFDVERNSCNNFIYGGCRGNKNSYRSEEACMLRCFRQQENPPLPLGSKVVLLAGLFVMVLI
  LFLGASMVYLIRVARRNQERALRTVWSSGDDKEQLVKNTYVL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_066925
• RefSeq Size: 1817
• RefSeq ORF: 756
• Synonyms: DIAR3; HAI-2; HAI2; Kop; PB
• Locus ID: 10653
• UniProt ID: O43291
• Cytogenetics: 19q13.2
• Summary: This gene encodes a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases. The protein inhibits HGF activator which prevents the formation of active hepatocyte growth factor. This gene is a putative tumor suppressor, and mutations in this gene result in congenital sodium diarrhea. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
• Protein Families: Transmembrane