TFPI (NM_001032281) Human Mass Spec Standard

CAT#: PH302098

TFPI MS Standard C13 and N15-labeled recombinant protein (NP_001027452)

Product Images

Coomassie blue staining of purified TFPI protein (Cat# TP302098). The protein was produced from HEK293T cells transfected with TFPI cDNA clone (Cat# RC202098) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC202098
• Predicted MW: 28.7 kDa
• Protein Sequence:
  Sequence Link

  >RC202098 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MIYTMKKVHALWASVCLLLNLAPAPLNADSEEDEEHTIITDTELPPLKLMHSFCAFKADDGPCKAIMKRF
  FFNIFTRQCEEFIYGGCEGNQNRFESLEECKKMCTRDNANRIIKTTLQQEKPDFCFLEEDPGICRGYITR
  YFYNNQTKQCERFKYGGCLGNMNNFETLEECKNICEDGPNGFQVDNYGTQLNAVNNSLTPQSTKVPSLFV
  TKEGTNDGWKNAAHIYQVFLNAFCIHASMFFLGLDSISCLC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001027452
• RefSeq Size: 1166
• RefSeq ORF: 753
• Synonyms: EPI; LACI; TFI; TFPI1
• Locus ID: 7035
• UniProt ID: P10646
• Cytogenetics: 2q32.1
• Summary: 'This gene encodes a Kunitz-type serine protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. Inhibition of the encoded protein restores hemostasis in animal models of hemophilia. This gene encodes multiple protein isoforms that differ in their inhibitory activity, specificity and cellular localization. [provided by RefSeq, Jul 2016]'
• Protein Families: Secreted Protein
• Protein Pathways: Complement and coagulation cascades