Frequenin (NCS1) (NM_014286) Human Mass Spec Standard

CAT#: PH302389

NCS1 MS Standard C13 and N15-labeled recombinant protein (NP_055101)

Product Images

Coomassie blue staining of purified NCS1 protein (Cat# TP302389). The protein was produced from HEK293T cells transfected with NCS1 cDNA clone (Cat# RC202389) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC202389
• Predicted MW: 21.9 kDa
• Protein Sequence:
  Sequence Link

  >RC202389 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGKSNSKLKPEVVEELTRKTYFTEKEVQQWYKGFIKDCPSGQLDAAGFQKIYKQFFPFGDPTKFATFVFN
  VFDENKDGRIEFSEFIQALSVTSRGTLDEKLRWAFKLYDLDNDGYITRNEMLDIVDAIYQMVGNTVELPE
  EENTPEKRVDRIFAMMDKNADGKLTLQEFQEGSKADPSIVQALSLYDGLV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_055101
• RefSeq Size: 5009
• RefSeq ORF: 570
• Synonyms: FLUP; FREQ
• Locus ID: 23413
• UniProt ID: P62166, A0A024R8B2
• Cytogenetics: 9q34.11
• Summary: This gene is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. The protein encoded by this gene regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. The protein is associated with secretory granules and modulates synaptic transmission and synaptic plasticity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Protein Families: Druggable Genome