Guanylate kinase (GUK1) (NM_000858) Human Mass Spec Standard

CAT#: PH302510

GUK1 MS Standard C13 and N15-labeled recombinant protein (NP_000849)

Product Images

Coomassie blue staining of purified GUK1 protein (Cat# TP302510). The protein was produced from HEK293T cells transfected with GUK1 cDNA clone (Cat# RC202510) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC202510
• Predicted MW: 21.7 kDa
• Protein Sequence:
  Sequence Link

  >RC202510 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSGPRPVVLSGPSGAGKSTLLKRLLQEHSGIFGFSVSHTTRNPRPGEENGKDYYFVTREVMQRDIAAGDF
  IEHAEFSGNLYGTSKVAVQAVQAMNRICVLDVDLQGVRNIKATDLRPIYISVQPPSLHVLEQRLRQRNTE
  TEESLVKRLAAAQADMESSKEPGLFDVVIINDSLDQAYAELKEALSEEIKKAQRTGA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_000849
• RefSeq Size: 1155
• RefSeq ORF: 591
• Synonyms: GMK
• Locus ID: 2987
• UniProt ID: Q16774, Q6IBG8
• Cytogenetics: 1q42.13
• Summary: 'The protein encoded by this gene is an enzyme that catalyzes the transfer of a phosphate group from ATP to guanosine monophosphate (GMP) to form guanosine diphosphate (GDP). The encoded protein is thought to be a good target for cancer chemotherapy. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2011]'
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Purine metabolism