BANF1 (NM_003860) Human Mass Spec Standard

CAT#: PH303270

BANF1 MS Standard C13 and N15-labeled recombinant protein (NP_003851)

Product Images

Coomassie blue staining of purified BANF1 protein (Cat# TP303270). The protein was produced from HEK293T cells transfected with BANF1 cDNA clone (Cat# RC203270) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC203270
• Predicted MW: 10.1 kDa
• Protein Sequence:
  Sequence Link

  >RC203270 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTTSQKHRDFVAEPMGEKPVGSLAGIGEVLGKKLEERGFDKAYVVLGQFLVLKKDEDLFREWLKDTCGAN
  AKQSRDCFGCLREWCDAFL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_003851
• RefSeq Size: 1179
• RefSeq ORF: 267
• Synonyms: BAF; BCRP1; D14S1460; NGPS
• Locus ID: 8815
• UniProt ID: O75531, A0A024R5H0
• Cytogenetics: 11q13.1
• Summary: The protein encoded by this gene was first identified by its ability to protect retroviruses from intramolecular integration and therefore promote intermolecular integration into the host cell genome. The protein forms a homodimer which localizes to both the nucleus and cytoplasm and is specifically associated with chromosomes during mitosis. This protein binds to double stranded DNA in a non-specific manner and also binds to LEM-domain containing proteins of the nuclear envelope. This protein is thought to facilitate nuclear reassembly by binding with both DNA and inner nuclear membrane proteins and thereby recruit chromatin to the nuclear periphery. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jan 2009]