NEK6 (NM_014397) Human Mass Spec Standard

CAT#: PH303609

NEK6 MS Standard C13 and N15-labeled recombinant protein (NP_055212)

Product Images

Coomassie blue staining of purified NEK6 protein (Cat# TP303609). The protein was produced from HEK293T cells transfected with NEK6 cDNA clone (Cat# RC203609) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC203609
• Predicted MW: 35.7 kDa
• Protein Sequence:
  Sequence Link

  >RC203609 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGQPGHMPHGGSSNNLCHTLGPVHPPDPQRHPNTLSFRCSLADFQIEKKIGRGQFSEVYKATCLLDRKT
  VALKKVQIFEMMDAKARQDCVKEIGLLKQLNHPNIIKYLDSFIEDNELNIVLELADAGDLSQMIKYFKKQ
  KRLIPERTVWKYFVQLCSAVEHMHSRRVMHRDIKPANVFITATGVVKLGDLGLGRFFSSETTAAHSLVGT
  PYYMSPERIHENGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLFSLCQKIEQCDYPPLPGEHYSEKLRE
  LVSMCICPDPHQRPDIGYVHQVAKQMHIWMSST
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_055212
• RefSeq Size: 2645
• RefSeq ORF: 939
• Synonyms: SID6-1512
• Locus ID: 10783
• UniProt ID: Q9HC98, A0A024R8A6
• Cytogenetics: 9q33.3
• Summary: The protein encoded by this gene is a kinase required for progression through the metaphase portion of mitosis. Inhibition of the encoded protein can lead to apoptosis. This protein also can enhance tumorigenesis by suppressing tumor cell senescence. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
• Protein Families: Druggable Genome, Protein Kinase