DTX2 (NM_020892) Human Mass Spec Standard

CAT#: PH303717

DTX2 MS Standard C13 and N15-labeled recombinant protein (NP_065943)

Product Images

Coomassie blue staining of purified DTX2 protein (Cat# TP303717). The protein was produced from HEK293T cells transfected with DTX2 cDNA clone (Cat# RC203717) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC203717
• Predicted MW: 67.2 kDa
• Protein Sequence:
  Sequence Link

  >RC203717 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAMAPSPSLVQVYTSPAAVAVWEWQDGLGTWHPYSATVCSFIEQQFVQQKGQRFGLGSLAHSIPLGQADP
  SLAPYIIDLPSWTQFRQDTGTMRAVRRHLFPQHSAPGRGVVWEWLSDDGSWTAYEASVCDYLEQQVARGN
  QLVDLAPLGYNYTVNYTTHTQTNKTSSFCRSVRRQAGPPYPVTTIIAPPGHTGVACSCHQCLSGSRTGPV
  SGRYRHSMTNLPAYPVPQHPPHRTASVFGTHQAFAPYNKPSLSGARSAPRLNTTNAWGAAPPSLGSQPLY
  RSSLSHLGPQHLPPGSSTSGAVSASLPSGPSSSPGSVPATVPMQMPKPSRVQQALAGMTSVLMSAIGLPV
  CLSRAPQPTSPPASRLASKSHGSVKRLRKMSVKGATPKPEPEPEQVIKNYTEELKVPPDEDCIICMEKLS
  AASGYSDVTDSKAIGSLAVGHLTKCSHAFHLLCLLAMYCNGNKDGSLQCPSCKTIYGEKTGTQPQGKMEV
  LRFQMSLPGHEDCGTILIVYSIPHGIQGPEHPNPGKPFTARGFPRQCYLPDNAQGRKVLELLKVAWKRRL
  IFTVGTSSTTGETDTVVWNEIHHKTEMDRNITGHGYPDPNYLQNVLAELAAQGVTEDCLEQQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_065943
• RefSeq Size: 2835
• RefSeq ORF: 1866
• Synonyms: RNF58
• Locus ID: 113878
• UniProt ID: Q86UW9
• Cytogenetics: 7q11.23
• Summary: DTX2 functions as an E3 ubiquitin ligase (Takeyama et al., 2003 [PubMed 12670957]). [supplied by OMIM, Nov 2009]
• Protein Families: Druggable Genome
• Protein Pathways: Notch signaling pathway