GLO1 (NM_006708) Human Mass Spec Standard

CAT#: PH303826

GLO1 MS Standard C13 and N15-labeled recombinant protein (NP_006699)

Product Images

Coomassie blue staining of purified GLO1 protein (Cat# TP303826). The protein was produced from HEK293T cells transfected with GLO1 cDNA clone (Cat# RC203826) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC203826
• Predicted MW: 20.8 kDa
• Protein Sequence:
  Sequence Link

  >RC203826 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAEPQPPSGGLTDEAALSYCSDADPSTKDFLLQQTMLRVKDPKKSLDFYTRVLGMTLIQKCDFPIMKFSL
  YFLAYEDKNDIPKEKDEKIAWALSRKATLELTHNWGTEDDETQSYHNGNSDPRGFGHIGIAVPDVYSACK
  RFEELGVKFVKKPDDGKMKGLAFIQDPDGYWIEILNPNKMATLM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_006699
• RefSeq Size: 2071
• RefSeq ORF: 552
• Synonyms: GLOD1; GLYI; HEL-S-74
• Locus ID: 2739
• UniProt ID: Q04760, X5DNM4
• Cytogenetics: 6p21.2
• Summary: 'The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq, Jul 2008]'
• Protein Pathways: Pyruvate metabolism