GLO1 (NM_006708) Human Recombinant Protein

CAT#: TP303826

Recombinant protein of human glyoxalase I (GLO1)

Product Images

Coomassie blue staining of purified GLO1 protein (Cat# TP303826). The protein was produced from HEK293T cells transfected with GLO1 cDNA clone (Cat# RC203826) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >RC203826 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAEPQPPSGGLTDEAALSYCSDADPSTKDFLLQQTMLRVKDPKKSLDFYTRVLGMTLIQKCDFPIMKFSL
  YFLAYEDKNDIPKEKDEKIAWALSRKATLELTHNWGTEDDETQSYHNGNSDPRGFGHIGIAVPDVYSACK
  RFEELGVKFVKKPDDGKMKGLAFIQDPDGYWIEILNPNKMATLM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-Myc/DDK
• Predicted MW: 20.6 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Note: For culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_006699
• Locus ID: 2739
• UniProt ID: Q04760, X5DNM4
• Cytogenetics: 6p21.2
• Refseq Size: 2071
• Refseq ORF: 552
• Synonyms: GLOD1; GLYI; HEL-S-74
• Summary: The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq, Jul 2008]
• Protein Pathways: Pyruvate metabolism