Nucleophosmin (NPM1) (NM_199185) Human Mass Spec Standard

CAT#: PH303841

NPM1 MS Standard C13 and N15-labeled recombinant protein (NP_954654)

Product Images

Coomassie blue staining of purified NPM1 protein (Cat# TP303841). The protein was produced from HEK293T cells transfected with NPM1 cDNA clone (Cat# RC203841) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC203841
• Predicted MW: 29.5 kDa
• Protein Sequence:
  Sequence Link

  >RC203841 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEDSMDMDMSPLRPQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEAMNYEGS
  PIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAVEEDAESEDEEEEDVKLLSISG
  KRSAPGGGSKVPQKKVKLAADEDDDDDDEEDDDEDDDDDDFDDEEAEEKAPVKKGQESFKKQEKTPKTPK
  GPSSVEDIKAKMQASIEKGGSLPKVEAKFINYVKNCFRMTDQEAIQDLWQWRKSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_954654
• RefSeq Size: 1362
• RefSeq ORF: 795
• Synonyms: B23; NPM
• Locus ID: 4869
• UniProt ID: P06748, A0A0S2Z4G7
• Cytogenetics: 5q35.1
• Summary: 'The protein encoded by this gene is involved in several cellular processes, including centrosome duplication, protein chaperoning, and cell proliferation. The encoded phosphoprotein shuttles between the nucleolus, nucleus, and cytoplasm, chaperoning ribosomal proteins and core histones from the nucleus to the cytoplasm. This protein is also known to sequester the tumor suppressor ARF in the nucleolus, protecting it from degradation until it is needed. Mutations in this gene are associated with acute myeloid leukemia. Dozens of pseudogenes of this gene have been identified. [provided by RefSeq, Aug 2017]'
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors