Ribonuclease H2, subunit A (RNASEH2A) (NM_006397) Human Mass Spec Standard

CAT#: PH304032

RNASEH2A MS Standard C13 and N15-labeled recombinant protein (NP_006388)

Product Images

Coomassie blue staining of purified RNASEH2A protein (Cat# TP304032). The protein was produced from HEK293T cells transfected with RNASEH2A cDNA clone (Cat# RC204032) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC204032
• Predicted MW: 33.4 kDa
• Protein Sequence:
  Sequence Link

  >RC204032 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDLSELERDNTGRCRLSSPVPAVCRKEPCVLGVDEAGRGPVLGPMVYAICYCPLPRLADLEALKVADSKT
  LLESERERLFAKMEDTDFVGWALDVLSPNLISTSMLGRVKYNLNSLSHDTATGLIQYALDQGVNVTQVFV
  DTVGMPETYQARLQQSFPGIEVTVKAKADALYPVVSAASICAKVARDQAVKKWQFVEKLQDLDTDYGSGY
  PNDPKTKAWLKEHVEPVFGFPQFVRFSWRTAQTILEKEAEDVIWEDSASENQEGLRKITSYFLNEGSQAR
  PRSSHRYFLERGLESATSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_006388
• RefSeq Size: 1148
• RefSeq ORF: 897
• Synonyms: AGS4; JUNB; RNASEHI; RNHIA; RNHL; THSD8
• Locus ID: 10535
• UniProt ID: O75792
• Cytogenetics: 19p13.13
• Summary: The protein encoded by this gene is a component of the heterotrimeric type II ribonuclease H enzyme (RNAseH2). RNAseH2 is the major source of ribonuclease H activity in mammalian cells and endonucleolytically cleaves ribonucleotides. It is predicted to remove Okazaki fragment RNA primers during lagging strand DNA synthesis and to excise single ribonucleotides from DNA-DNA duplexes. Mutations in this gene cause Aicardi-Goutieres Syndrome (AGS), a an autosomal recessive neurological disorder characterized by progressive microcephaly and psychomotor retardation, intracranial calcifications, elevated levels of interferon-alpha and white blood cells in the cerebrospinal fluid. [provided by RefSeq, Aug 2009]
• Protein Pathways: DNA replication