PPCDC (NM_021823) Human Mass Spec Standard

CAT#: PH304085

PPCDC MS Standard C13 and N15-labeled recombinant protein (NP_068595)

Product Images

Coomassie blue staining of purified PPCDC protein (Cat# TP304085). The protein was produced from HEK293T cells transfected with PPCDC cDNA clone (Cat# RC204085) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC204085
• Predicted MW: 22.4 kDa
• Protein Sequence:
  Sequence Link

  >RC204085 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEPKASCPAAAPLMERKFHVLVGVTGSVAALKLPLLVSKLLDIPGLEVAVVTTERAKHFYSPQDIPVTLY
  SDADEWEMWKSRSDPVLHIDLRRWADLLLVAPLDANTLGKVASGICDNLLTCVMRAWDRSKPLLFCPAMN
  TAMWEHPITAQQVDQLKAFGYVEIPCVAKKLVCGDEGLGAMAEVGTIVDKVKEVLFQHSGFQQS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_068595
• RefSeq Size: 2268
• RefSeq ORF: 612
• Synonyms: coaC; MDS018; PPC-DC
• Locus ID: 60490
• UniProt ID: Q96CD2
• Cytogenetics: 15q24.2
• Summary: Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCDC (EC 4.1.1.36), one of the last enzymes in this pathway, converts phosphopantothenoylcysteine to 4-prime-phosphopantetheine (Daugherty et al., 2002 [PubMed 11923312]). [supplied by OMIM, Mar 2008]
• Protein Pathways: Metabolic pathways, Pantothenate and CoA biosynthesis