PPCDC (NM_021823) Human Tagged ORF Clone
CAT#: RC204085
PPCDC (Myc-DDK-tagged)-Human phosphopantothenoylcysteine decarboxylase (PPCDC)
"NM_021823" in other vectors (5)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | PPCDC |
Synonyms | coaC; MDS018; PPC-DC |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC204085 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACCAAAGGCCTCCTGTCCAGCTGCTGCACCCTTGATGGAGAGAAAATTCCATGTTCTTGTGGGTG TCACGGGGAGTGTCGCAGCCCTGAAGTTGCCTCTTCTGGTGTCAAAGCTTTTGGACATTCCTGGGCTGGA AGTAGCAGTGGTCACAACTGAGAGAGCCAAACATTTCTACAGCCCCCAGGACATTCCTGTCACCCTCTAC AGCGACGCTGATGAATGGGAGATGTGGAAGAGCCGCTCTGACCCAGTTCTGCACATTGACCTGCGGAGGT GGGCAGACCTCCTGCTGGTGGCTCCTCTTGATGCCAACACTCTGGGGAAGGTGGCCAGTGGCATCTGTGA CAACTTGCTTACCTGCGTCATGCGGGCCTGGGACCGCAGCAAGCCCCTGCTCTTCTGCCCGGCCATGAAC ACCGCCATGTGGGAGCACCCGATCACAGCGCAGCAGGTAGACCAGCTCAAGGCCTTTGGCTATGTCGAGA TCCCCTGTGTGGCCAAGAAGCTGGTGTGCGGAGATGAAGGTCTCGGGGCCATGGCTGAAGTGGGGACCAT CGTGGACAAAGTGAAAGAAGTCCTCTTCCAGCACAGTGGCTTCCAGCAGAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204085 protein sequence
Red=Cloning site Green=Tags(s) MEPKASCPAAAPLMERKFHVLVGVTGSVAALKLPLLVSKLLDIPGLEVAVVTTERAKHFYSPQDIPVTLY SDADEWEMWKSRSDPVLHIDLRRWADLLLVAPLDANTLGKVASGICDNLLTCVMRAWDRSKPLLFCPAMN TAMWEHPITAQQVDQLKAFGYVEIPCVAKKLVCGDEGLGAMAEVGTIVDKVKEVLFQHSGFQQS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_021823 |
ORF Size | 612 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021823.2, NM_021823.3, NM_021823.4, NP_068595.3 |
RefSeq Size | 2268 bp |
RefSeq ORF | 615 bp |
Locus ID | 60490 |
Domains | Flavoprotein |
Protein Pathways | Metabolic pathways, Pantothenate and CoA biosynthesis |
MW | 22.4 kDa |
Gene Summary | Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCDC (EC 4.1.1.36), one of the last enzymes in this pathway, converts phosphopantothenoylcysteine to 4-prime-phosphopantetheine (Daugherty et al., 2002 [PubMed 11923312]). [supplied by OMIM, Mar 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC317303 | PPCDC (untagged)-Human phosphopantothenoylcysteine decarboxylase (PPCDC) |
USD 420.00 |
|
SC319643 | PPCDC (untagged)-Human phosphopantothenoylcysteine decarboxylase (PPCDC) |
USD 420.00 |
|
RG204085 | PPCDC (GFP-tagged) - Human phosphopantothenoylcysteine decarboxylase (PPCDC) |
USD 460.00 |
|
RC204085L3 | Lenti ORF clone of Human phosphopantothenoylcysteine decarboxylase (PPCDC), Myc-DDK-tagged |
USD 620.00 |
|
RC204085L4 | Lenti ORF clone of Human phosphopantothenoylcysteine decarboxylase (PPCDC), mGFP tagged |
USD 620.00 |
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