CDK2AP1 (NM_004642) Human Mass Spec Standard

CAT#: PH304442

CDK2AP1 MS Standard C13 and N15-labeled recombinant protein (NP_004633)

Product Images

Coomassie blue staining of purified CDK2AP1 protein (Cat# TP304442). The protein was produced from HEK293T cells transfected with CDK2AP1 cDNA clone (Cat# RC204442) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC204442
• Predicted MW: 12.4 kDa
• Protein Sequence:
  Sequence Link

  >RC204442 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSYKPNLAAHMPAAALNAAGSVHSPSTSMATSSQYRQLLSDYGPPSLGYTQGTGNSQVPQSKYAELLAII
  EELGKEIRPTYAGSKSAMERLKRGIIHARGLVRECLAETERNARS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_004633
• RefSeq Size: 1655
• RefSeq ORF: 345
• Synonyms: doc-1; DOC1; DORC1; p12DOC-1; ST19
• Locus ID: 8099
• UniProt ID: O14519
• Cytogenetics: 12q24.31
• Summary: The protein encoded by this gene is a cyclin-dependent kinase 2 (CDK2) -associated protein which is thought to negatively regulate CDK2 activity by sequestering monomeric CDK2, and targeting CDK2 for proteolysis. This protein was found to also interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggests a regulatory role in DNA replication during the S-phase of the cell cycle. This protein also forms a core subunit of the nucleosome remodeling and histone deacetylation (NURD) complex that epigenetically regulates embryonic stem cell differentiation. This gene thus plays a role in both cell-cycle and epigenetic regulation. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]