CHMP4C (NM_152284) Human Mass Spec Standard

CAT#: PH304802

CHMP4C MS Standard C13 and N15-labeled recombinant protein (NP_689497)

Product Images

Coomassie blue staining of purified CHMP4C protein (Cat# TP304802). The protein was produced from HEK293T cells transfected with CHMP4C cDNA clone (Cat# RC204802) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC204802
• Predicted MW: 26.2 kDa
• Protein Sequence:
  Sequence Link

  >RC204802 representing NM_152284
  Red=Cloning site Green=Tags(s)
  
  MSKLGKFFKGGGSSKSRAAPSPQEALVRLRETEEMLGKKQEYLENRIQREIALAKKHGTQNKRAALQALK
  RKKRFEKQLTQIDGTLSTIEFQREALENSHTNTEVLRNMGFAAKAMKSVHENMDLNKIDDLMQEITEQQD
  IAQEISEAFSQRVGFGDDFDEDELMAELEELEQEELNKKMTNIRLPNVPSSSLPAQPNRKPGMSSTARRS
  RAASSQRAEEEDDDIKQLAAWAT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_689497
• RefSeq Size: 1847
• RefSeq ORF: 699
• Synonyms: Shax3; SNF7-3; VPS32C
• Locus ID: 92421
• UniProt ID: Q96CF2
• Cytogenetics: 8q21.13
• Summary: CHMP4C belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]). [supplied by OMIM, Mar 2008]
• Protein Pathways: Endocytosis