Osteopontin (SPP1) (NM_001040058) Human Mass Spec Standard

CAT#: PH304803

SPP1 MS Standard C13 and N15-labeled recombinant protein (NP_001035147)

Product Images

Coomassie blue staining of purified SPP1 protein (Cat# TP304803). The protein was produced from HEK293T cells transfected with SPP1 cDNA clone (Cat# RC204803) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC204803
• Predicted MW: 35.4 kDa
• Protein Sequence:
  Sequence Link

  >RC204803 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRIAVICFCLLGITCAIPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQKQNLLAPQNAVSSEETNDFK
  QETLPSKSNESHDHMDDMDDEDDDDHVDSQDSIDSNDSDDVDDTDDSHQSDESHHSDESDELVTDFPTDL
  PATEVFTPVVPTVDTYDGRGDSVVYGLRSKSKKFRRPDIQYPDATDEDITSHMESEELNGAYKAIPVAQD
  LNAPSDWDSRGKDSYETSQLDDQSAETHSHKQSRLYKRKANDESNEHSDVIDSQELSKVSREFHSHEFHS
  HEDMLVVDPKSKEEDKHLKFRISHELDSASSEVN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001035147
• RefSeq Size: 1641
• RefSeq ORF: 942
• Synonyms: BNSP; BSPI; ETA-1; OPN
• Locus ID: 6696
• UniProt ID: P10451, A0A024RDE2
• Cytogenetics: 4q22.1
• Summary: 'The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]'
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: ECM-receptor interaction, Focal adhesion, Toll-like receptor signaling pathway