Osteopontin (SPP1) (NM_001040058) Human Recombinant Protein

CAT#: TP710213

Purified protein of Homo sapiens secreted phosphoprotein 1 (SPP1), transcript variant 1, residues 17-314aa, secretory expressed with GP67 signal peptide, with C-terminal DDK tag, expressed in sf9, 20ug

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: Sf9
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  A DNA sequence from TrueORF clone, RC204803, encoding the region(Met-Ile17-Asn314) of Homo sapiens SPP1
• Tag: C-DDK
• Predicted MW: 33.7 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 50mM Tris-HCl, pH8.0, 100mM glycine, 10% glycerol.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_001035147
• Locus ID: 6696
• UniProt ID: P10451, A0A024RDE2
• Cytogenetics: 4q22.1
• Refseq Size: 1641
• Refseq ORF: 942
• Synonyms: BNSP; BSPI; ETA-1; OPN
• Summary: 'The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]'
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: ECM-receptor interaction, Focal adhesion, Toll-like receptor signaling pathway