DTX3L (NM_138287) Human Mass Spec Standard

CAT#: PH307944

DTX3L MS Standard C13 and N15-labeled recombinant protein (NP_612144)

Product Images

Coomassie blue staining of purified DTX3L protein (Cat# TP307944). The protein was produced from HEK293T cells transfected with DTX3L cDNA clone (Cat# RC207944) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC207944
• Predicted MW: 83.6 kDa
• Protein Sequence:
  Sequence Link

  >RC207944 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASHLRPPSPLLVRVYKSGPRVRRKLESYFQSSKSSGGGECTVSTQEHEAPGTFRVEFSERAAKERVLKK
  GEHQILVDEKPVPIFLVPTENSIKKNTRPQISSLTQSQAETPSGDMHQHEGHIPNAVDSCLQKIFLTVTA
  DLNCNLFSKEQRAYITTLCPSIRKMEGHDGIEKVCGDFQDIERIHQFLSEQFLESEQKQQFSPSMTERKP
  LSQQERDSCISPSEPETKAEQKSNYFEVPLPYFEYFKYICPDKINSIEKRFGVNIEIQESSPNMVCLDFT
  SSRSGDLEAARESFASEFQKNTEPLKQECVSLADSKQANKFKQELNHQFTKLLIKEKGGELTLLGTQDDI
  SAAKQKISEAFVKIPVKLFAANYMMNVIEVDSAHYKLLETELLQEISEIEKRYDICSKVSEKGQKTCILF
  ESKDRQVDLSVHAYASFIDAFQHASCQLMREVLLLKSLGKERKHLHQTKFADDFRKRHPNVHFVLNQESM
  TLTGLPNHLAKAKQYVLKGGGMSSLAGKKLKEGHETPMDIDSDDSKAASPPLKGSVSSEASELDKKEKGI
  CVICMDTISNKKVLPKCKHEFCAPCINKAMSYKPICPTCQTSYGIQKGNQPEGSMVFTVSRDSLPGYESF
  GTIVITYSMKAGIQTEEHPNPGKRYPGIQRTAYLPDNKEGRKVLKLLYRAFDQKLIFTVGYSRVLGVSDV
  ITWNDIHHKTSRFGGPEMYGYPDPSYLKRVKEELKAKGIE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_612144
• RefSeq Size: 5768
• RefSeq ORF: 2220
• Synonyms: BBAP; RNF143
• Locus ID: 151636
• UniProt ID: Q8TDB6
• Cytogenetics: 3q21.1
• Summary: DTX3L functions as an E3 ubiquitin ligase (Takeyama et al., 2003 [PubMed 12670957]). [supplied by OMIM, Nov 2009]
• Protein Families: Druggable Genome
• Protein Pathways: Notch signaling pathway