Ferritin Heavy Chain (FTH1) (NM_002032) Human Mass Spec Standard

CAT#: PH309845

FTH1 MS Standard C13 and N15-labeled recombinant protein (NP_002023)

Product Images

Coomassie blue staining of purified FTH1 protein (Cat# TP309845). The protein was produced from HEK293T cells transfected with FTH1 cDNA clone (Cat# RC209845) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC209845
• Predicted MW: 21.2 kDa
• Protein Sequence:
  Sequence Link

  >RC209845 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTTASTSQVRQNYHQDSEAAINRQINLELYASYVYLSMSYYFDRDDVALKNFAKYFLHQSHEEREHAEKL
  MKLQNQRGGRIFLQDIKKPDCDDWESGLNAMECALHLEKNVNQSLLELHKLATDKNDPHLCDFIETHYLN
  EQVKAIKELGDHVTNLRKMGAPESGLAEYLFDKHTLGDSDNES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_002023
• RefSeq Size: 1245
• RefSeq ORF: 549
• Synonyms: FHC; FTH; FTHL6; HFE5; PIG15; PLIF
• Locus ID: 2495
• UniProt ID: P02794, A0A024R525
• Cytogenetics: 11q12.3
• Summary: 'This gene encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]'
• Protein Families: Druggable Genome
• Protein Pathways: Porphyrin and chlorophyll metabolism