Proteasome subunit beta type 2 (PSMB2) (NM_002794) Human Mass Spec Standard

CAT#: PH310452

PSMB2 MS Standard C13 and N15-labeled recombinant protein (NP_002785)

Product Images

Coomassie blue staining of purified PSMB2 protein (Cat# TP310452). The protein was produced from HEK293T cells transfected with PSMB2 cDNA clone (Cat# RC210452) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC210452
• Predicted MW: 22.8 kDa
• Protein Sequence:
  Sequence Link

  >RC210452 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEYLIGIQGPDYVLVASDRVAASNIVQMKDDHDKMFKMSEKILLLCVGEAGDTVQFAEYIQKNVQLYKMR
  NGYELSPTAAANFTRRNLADCLRSRTPYHVNLLLAGYDEHEGPALYYMDYLAALAKAPFAAHGYGAFLTL
  SILDRYYTPTISRERAVELLRKCLEELQKRFILNLPTFSVRIIDKNGIHDLDNISFPKQGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_002785
• RefSeq Size: 4759
• RefSeq ORF: 603
• Synonyms: HC7-I
• Locus ID: 5690
• UniProt ID: P49721, A0A140VJS6
• Cytogenetics: 1p34.3
• Summary: 'The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]'
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Proteasome