NIPP1 (PPP1R8) (NM_138558) Human Mass Spec Standard

CAT#: PH310575

PPP1R8 MS Standard C13 and N15-labeled recombinant protein (NP_612568)

Product Images

Coomassie blue staining of purified PPP1R8 protein (Cat# TP310575). The protein was produced from HEK293T cells transfected with PPP1R8 cDNA clone (Cat# RC210575) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC210575
• Predicted MW: 38.5 kDa
• Protein Sequence:
  Sequence Link

  >RC210575 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAAANSGSSLPLFDCPTWAGKPPPGLHLDVVKGDKLIEKLIIDEKKYYLFGRNPDLCDFTIDHQSCSRV
  HAALVYHKHLKRVFLIDLNSTHGTFLGHIRLEPHKPQQIPIDSTVSFGASTRAYTLREKPQTLPSAVKGD
  EKMGGEDDELKGLLGLPEEETELDNLTEFITAHNKRISTLTIEEGNLDIQRPKRKRKNSRVTFSEDDEII
  NPEDVDPSVGRFRNMVQTAVVPVKKKRVEGPGSLGLEESGSRRMQNFAFSGGLYGGLPPTHSEAGSQPHG
  IHGTALIGGLPMPYPNLAPDVDLTPVVPSAVNMNPAPNPAVYNPEAVNEPKKKKYAKEAWPGKKPTPSLL
  I
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_612568
• RefSeq Size: 2659
• RefSeq ORF: 1056
• Synonyms: ARD-1; ARD1; NIPP-1; NIPP1; PRO2047
• Locus ID: 5511
• UniProt ID: Q12972
• Cytogenetics: 1p35.3
• Summary: 'This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. [provided by RefSeq, Jul 2008]'
• Protein Families: Druggable Genome, Transcription Factors