NIPP1 (PPP1R8) (NM_138558) Human Recombinant Protein

CAT#: TP310575

Recombinant protein of human protein phosphatase 1, regulatory (inhibitor) subunit 8 (PPP1R8), transcript variant 2

Product Images

Coomassie blue staining of purified PPP1R8 protein (Cat# TP310575). The protein was produced from HEK293T cells transfected with PPP1R8 cDNA clone (Cat# RC210575) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >RC210575 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAAANSGSSLPLFDCPTWAGKPPPGLHLDVVKGDKLIEKLIIDEKKYYLFGRNPDLCDFTIDHQSCSRV
  HAALVYHKHLKRVFLIDLNSTHGTFLGHIRLEPHKPQQIPIDSTVSFGASTRAYTLREKPQTLPSAVKGD
  EKMGGEDDELKGLLGLPEEETELDNLTEFITAHNKRISTLTIEEGNLDIQRPKRKRKNSRVTFSEDDEII
  NPEDVDPSVGRFRNMVQTAVVPVKKKRVEGPGSLGLEESGSRRMQNFAFSGGLYGGLPPTHSEAGSQPHG
  IHGTALIGGLPMPYPNLAPDVDLTPVVPSAVNMNPAPNPAVYNPEAVNEPKKKKYAKEAWPGKKPTPSLL
  I
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-Myc/DDK
• Predicted MW: 22.5 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Note: For culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_612568
• Locus ID: 5511
• UniProt ID: Q12972
• Cytogenetics: 1p35.3
• Refseq Size: 2659
• Refseq ORF: 1056
• Synonyms: ARD-1; ARD1; NIPP-1; NIPP1; PRO2047
• Summary: This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. [provided by RefSeq, Jul 2008]
• Protein Families: Druggable Genome, Transcription Factors