ISCU (NM_014301) Human Mass Spec Standard

CAT#: PH312031

ISCU MS Standard C13 and N15-labeled recombinant protein (NP_055116)

Product Images

Coomassie blue staining of purified ISCU protein (Cat# TP312031). The protein was produced from HEK293T cells transfected with ISCU cDNA clone (Cat# RC212031) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC212031
• Predicted MW: 15.1 kDa
• Protein Sequence:
  Sequence Link

  >RC212031 representing NM_014301
  Red=Cloning site Green=Tags(s)
  
  MVLIDMSVDLSTQVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGC
  GSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCSMLAEDAIKAALADYKLKQEPKKGEAE
  KK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_055116
• RefSeq Size: 1086
• RefSeq ORF: 426
• Synonyms: 2310020H20Rik; HML; hnifU; ISU2; NIFU; NIFUN
• Locus ID: 23479
• UniProt ID: Q9H1K1, A0A024RBI3, B3KQ30
• Cytogenetics: 12q23.3
• Summary: This gene encodes a component of the iron-sulfur (Fe-S) cluster scaffold. Fe-S clusters are cofactors that play a role in the function of a diverse set of enzymes, including those that regulate metabolism, iron homeostasis, and oxidative stress response. Alternative splicing results in transcript variants encoding different protein isoforms that localize either to the cytosol or to the mitochondrion. Mutations in this gene have been found in patients with hereditary myopathy with lactic acidosis. A disease-associated mutation in an intron may activate a cryptic splice site, resulting in the production of a splice variant encoding a putatively non-functional protein. A pseudogene of this gene is present on chromosome 1. [provided by RefSeq, Feb 2016]