p16 ARC (ARPC5) (NM_005717) Human Mass Spec Standard

CAT#: PH312631

ARPC5 MS Standard C13 and N15-labeled recombinant protein (NP_005708)

Product Images

Coomassie blue staining of purified ARPC5 protein (Cat# TP312631). The protein was produced from HEK293T cells transfected with ARPC5 cDNA clone (Cat# RC212631) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC212631
• Predicted MW: 16.1 kDa
• Protein Sequence:
  Sequence Link

  >RC212631 representing NM_005717
  Red=Cloning site Green=Tags(s)
  
  MSKNTVSSARFRKVDVDEYDENKFVDEEDGGDGQAGPDEGEVDSCLRQGNMTAALQAALKNPPINTKSQA
  VKDRAGSIVLKVLISFKANDIEKAVQSLDKNGVDLLMKYIYKGFESPSDNSSAMLLQWHEKALAAGGVGS
  IVRVLTARKTV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_005708
• RefSeq Size: 2000
• RefSeq ORF: 453
• Synonyms: ARC16; dJ127C7.3; p16-Arc
• Locus ID: 10092
• UniProt ID: O15511
• Cytogenetics: 1q25.3
• Summary: This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p16 subunit, has yet to be determined. Alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]
• Protein Pathways: Fc gamma R-mediated phagocytosis, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton