SPIRE1 (NM_020148) Human Mass Spec Standard

CAT#: PH313848

SPIRE1 MS Standard C13 and N15-labeled recombinant protein (NP_064533)

Product Images

Coomassie blue staining of purified SPIRE1 protein (Cat# TP313848). The protein was produced from HEK293T cells transfected with SPIRE1 cDNA clone (Cat# RC213848) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC213848
• Predicted MW: 67.1 kDa
• Protein Sequence:
  Sequence Link

  >RC213848 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MANTVEADGSNDEGYEAAEEGLGDEDEKRKISAIRSYRDVMKLCAAHLPTESDAPNHYQAVCRALFAETM
  ELHTFLTKIKSAKENLKKIQEMEKSDESSTDLEELKNADWARFWVQVMRDLRNGVKLKKVQERQYNPLPI
  EYQLTPYEMLMDDIRCKRYTLRKVMVNGDIPPRLKKSAHEIILDFIRSRPPLNPVSARKLKPTPPRPRSL
  HERILEEIKAERKLRPVSPEEIRRSRLDVTTPESTKNLVESSMVNGGLTSQTKENGLSTSQQVPAQRKKL
  LRAPTLAELDSSESEEETLHKSTSSSSVSPSFPEEPVLEAVSTRKKPPKFLPISSTPQPERRQPPQRRHS
  IEKETPTNVRQFLPPSRQSSRSLEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDIYTALKKGKL
  CFCCRTRRFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESSMRSEKPSTAHH
  RPLRSIARFSSKSKSMDKSDEELQFPKELMEDWSTMEVCVDCKKFISEIISSSRRSLVLANKRARLKRKT
  QSFYMSSPGPSEYCPSERTISEI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_064533
• RefSeq Size: 5402
• RefSeq ORF: 1749
• Synonyms: Spir-1
• Locus ID: 56907
• UniProt ID: Q08AE8
• Cytogenetics: 18p11.21
• Summary: Spire proteins, such as SPIRE1, are highly conserved between species. They belong to the family of Wiskott-Aldrich homology region-2 (WH2) proteins, which are involved in actin organization (Kerkhoff et al., 2001 [PubMed 11747823]). [supplied by OMIM, Mar 2008]
• Protein Pathways: Dorso-ventral axis formation