SMYD2 (NM_020197) Human Mass Spec Standard

CAT#: PH314307

SMYD2 MS Standard C13 and N15-labeled recombinant protein (NP_064582)

Product Images

Coomassie blue staining of purified SMYD2 protein (Cat# TP314307). The protein was produced from HEK293T cells transfected with SMYD2 cDNA clone (Cat# RC214307) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC214307
• Predicted MW: 49.5 kDa
• Protein Sequence:
  Sequence Link

  >RC214307 representing NM_020197
  Red=Cloning site Green=Tags(s)
  
  MRAEGLGGLERFCSPGKGRGLRALQPFQVGDLLFSCPAYAYVLTVNERGNHCEYCFTRKEGLSKCGRCKQ
  AFYCNVECQKEDWPMHKLECSPMVVFGENWNPSETVRLTARILAKQKIHPERTPSEKLLAVKEFESHLDK
  LDNEKKDLIQSDIAALHHFYSKHLEFPDNDSLVVLFAQVNCNGFTIEDEELSHLGSAIFPDVALMNHSCC
  PNVIVTYKGTLAEVRAVQEIKPGEEVFTSYIDLLYPTEDRNDRLRDSYFFTCECQECTTKDKDKAKVEIR
  KLSDPPKAEAIRDMVRYARNVIEEFRRAKHYKSPSELLEICELSQEKMSSVFEDSNVYMLHMMYQAMGVC
  LYMQDWEGALQYGQKIIKPYSKHYPLYSLNVASMWLKLGRLYMGLEHKAAGEKALKKAIAIMEVAHGKDH
  PYISEIKQEIESH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_064582
• RefSeq Size: 1694
• RefSeq ORF: 1299
• Synonyms: HSKM-B; KMT3C; ZMYND14
• Locus ID: 56950
• UniProt ID: Q9NRG4
• Cytogenetics: 1q32.3
• Summary: SET domain-containing proteins, such as SMYD2, catalyze lysine methylation (Brown et al., 2006 [PubMed 16805913]). [supplied by OMIM, Nov 2008]
• Protein Families: Druggable Genome