Calpain 9 (CAPN9) (NM_006615) Human Mass Spec Standard

CAT#: PH315171

CAPN9 MS Standard C13 and N15-labeled recombinant protein (NP_006606)

Product Images

Coomassie blue staining of purified CAPN9 protein (Cat# TP315171). The protein was produced from HEK293T cells transfected with CAPN9 cDNA clone (Cat# RC215171) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC215171
• Predicted MW: 78.9 kDa
• Protein Sequence:
  Sequence Link

  >RC215171 representing NM_006615
  Red=Cloning site Green=Tags(s)
  
  MPYLYRAPGPQAHPVPKDARITHSSGQSFEQMRQECLQRGTLFEDADFPASNSSLFYSERPQIPFVWKRP
  GEIVKNPEFILGGATRTDICQGELGDCWLLAAIASLTLNQKALARVIPQDQRFGPGYAGIFHFQFWQHSE
  WLDVVIDDRLPTFRDRLVFLHSADHNEFWSALLEKAYAKLNGSYEALKGGSAIEAMEDFTGGVAETFQTK
  EAPENFYEILEKALKRGSLLGCFIDTRSAAESEARTPFGLIKGHAYSVTGIDQVSFRGQRIELIRIRNPW
  GQVEWNGSWSDSSPEWRSVGPAEQKRLCHTALDDGEFWMAFQDFKAHFDKVEICNLTPDALEEDAIHKWE
  VTVHQGSWVRGSTAGGCRNFLDTFWTNPQIKLSLTEKDEGQEECSFLVALMQKDRRKLKRFGANVLTIGY
  AIYECPDKDEHLNKDFFRYHASRARSKTFINLREVSDRFKLPPGEYILIPSTFEPHQEADFCLRIFSEKK
  AITRDMDGNVDIDLPEPPKPTPPDQETEEEQRFRALFEQVAGEDMEVTAEELEYVLNAVLQKKKDIKFKK
  LSLISCKNIISLMDTSGNGKLEFDEFKVFWDKLKQWINLFLRFDADKSGTMSTYELRTALKAAGFQLSSH
  LLQLIVLRYADEELQLDFDDFLNCLVRLENASRVFQALSTKNKEFIHLNINEFIHLTMNI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_006606
• RefSeq Size: 2362
• RefSeq ORF: 2070
• Synonyms: GC36; nCL-4
• Locus ID: 10753
• UniProt ID: O14815, Q6PIV8
• Cytogenetics: 1q42.2
• Summary: Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. The protein encoded by this gene is expressed predominantly in stomach and small intestine and may have specialized functions in the digestive tract. This gene is thought to be associated with gastric cancer. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Protein Families: Druggable Genome, Protease